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Requisition Number 16-0162
Post Date 9/12/2017
Title Biology Technician
Position Type Full Time
Work Hours 40 hpw
City Morgantown
State WV
Description Purpose
The primary purpose of this position is to support the Principal Investigator (PI)/Core Facility Director as a technician performing a wide variety of molecular and cell biology experiments and to lead or actively participate in a research project initiated in the lab. This position will advance the lab’s studies and provide crucial data that can be used for future research opportunities. Incumbent will be responsible for the design and conduction of experiments, as well as provide daily oversight of the lab.

This position provides program, as well as direct internal and external client support services. Responds to inquiries and independently resolves matters of low to moderate complexity. Responsibilities require some judgment, resourcefulness, and originality, but diverse guides and precedents are usually available to apply or adapt to the circumstances. Work effort has considerable impact on client services, program and project image, with potential legal liabilities.

Duties & Responsibilities
Responsibilities are functional in nature, and performed under general supervision. Specific tasks include, but are not limited to:
1) Performs a variety of molecular and cell biology experiments to conduct cancer research projects in the area of signaling and tumor models. These may include:
a. Culture and manipulation of cells - culture of bacteria and mammalian cells, generation of stably expressing cell lines, transfection/transformation of mammalian, bacteria cells, organoid and 3D-matrix primary cell culture, cell based assays on proliferation, death, survival.
b. Analysis of genes - cloning of genes (cDNAs) by different methods, PCR amplification, generation of mutant genes (cDNAs), analysis of DNA/RNA sequencing results, preparation of samples for RNA and whole genome sequencing.
c. Analysis of proteins - expression and purification of proteins from bacterial and mammalian cells, isolation of protein lysates, analysis of proteins by SDS/PAGE, immunoblot, protein staining, identification and characterization of protein complexes, immunoprecipitation, co-immunoprecipitation, immunofluorescence analysis, cytometry analysis, immunohistochemistry, of protein expression of tissues.
d. Tissue analysis - tissue analysis include but no limited to tissue procurement, fixation, analysis using in situ hybridization, immunohistochemistry, microscopy.
e. Microscopy-based analysis of cells and tissue involves, usage of confocal and epi-fluorescent microscopes; live imaging of cells, electron microscopy of fixed tissue.
f. Mice - performing experiments on and handling of mice including breeding, colony management, survival surgery, tumor transplantation, necropsy, and genotyping.
g. Collection and recording of data - keeping detailed records on all performed experiments; collect, analyze and present data using basic computer-based programs such as Excel, Word, PowerPoint. Collection and inventory tracking of lab resources, management and archiving of basic lab tools. Keeping animal inventory, health records and up to date policy records.
2) Assists the PI and coordinates tasks for students and other staff within the lab setting. May be responsible for assisting with training on area(s) of expertise to new individuals on proper procedures, techniques, equipment usage, etc.
3) Assists PI in running all aspects of the day to day operations within the laboratory. Makes some administrative decisions regarding experiments and will be responsible for learning all aspects of the lab procedures and equipment and provide training to others as needed.
4) Maintenance of the laboratory, laboratory equipment, glass ware, reagents.
5) Keeps accurate inventory.
6) Makes reagents and solution as necessary.
7) Serves as primary contact for vendors to order supplies or equipment repairs as necessary.
8) Performs or assists in research, participates in lab meetings and serves on other committees as needed.
9) Performs manual or computer-assisted data analysis.
10) Assists with writing research papers, reports, articles for journals in order to contribute to and be listed as a co-author on publications, which will help contribute to the reputation of WVU as a major research institution.
11) Collaborates with other highly prestigious investigators both internally and at other institutions to gather and share common research data techniques in order to design new procedures for own research.
12) Performs literature searches, assists with writing protocol for PI.
13) Maintain personal growth and development by attending courses or seminars, subject to PI’s approval.
14) Manages quality assessment documents and databases.
15) Actively participates in lab meetings.
16) Assists PI on a regular basis to plan experiments, discuss obtained results, and all aspects that concern the laboratory. Designs strategies to obtain research objectives and keeps accurate lab books/reports. Provides training to others on these matters.
17) Ensures compliance with University guidelines for research by reviewing safety procedures, animal protocols, chemical hygiene plan, monitoring the handling and disposal of hazardous materials and developing internal methods for maintaining a safe working environment.
18) Completes other general administrative/laboratory research duties as assigned by the PI.

Supervisory/Lead Role
Reports to the Principle Investigator. Normally receives minimal instructions on routine work, and general instructions on new assignments. Supervision of others is not required; however, sharing of knowledge with others and team participation is expected in the role. Program, project or activity lead responsibilities will occur or be assigned in this position.

Physical Demands
Activities require manual skill to perform accurate micropipetting of samples, mixing of small volumes of DNA/RNA, standing sitting by the bench, lifting of fluid-containing carboys, bending, carrying lightweight objects (up to 40 lbs), and transporting mice from laboratory to animal facility, traversing stairs or using elevators. Good hand-eye coordination, precision of movements, and attention to detail is required.

Work Environment
Work will be performed in a standard laboratory environment including using computers and laboratory equipment with electrical connections. Some work will be performed in the cold room and some laboratory chemicals have an odor or toxic fumes. Some work will be performed in the dark room either under dim red light or in the complete darkness using night vision equipment. There will be exposure to human, murine tissues, laboratory animals and low levels of radiation, however, protective measures will be provided.

The following items have been determined as possible job exposures of this position requiring WVU EHS medical monitoring:
1) Animal Contact – direct exposure to vertebrate animals, animal tissues, or research with body fluids or wastes.
2) Contacting Hazardous Chemicals – daily or weekly, using strong acids or caustics, fuel or tar products or cancer-causing chemicals.
3) Contact with Human Blood or Other Potentially Infectious Material (OPIM) – includes human body fluids other than feces, urine tears and sweat.
4) Respirator Use (Voluntary) – for individuals who wear disposable masks or medical procedure masks.
5) Scientific Research – research with nanoparticles, lasers, anesthetic, fuels or mining, aquatic or diving.
Requirements Requisite Qualifications
Education and/or Experience
1) Bachelor’s degree in physiology, biochemistry, biology or related field;
2) 2 – 4 years of related research experience
3) An equivalent combination of education and/or experience.

Skills, Knowledge, Abilities
1) Knowledge of laboratory environment and appropriate procedures.
2) Strong time management and work load skills.
3) Skilled at assessing chemical concentrations/amounts of various chemical components in solutions.
4) Skilled at organizing information and preparing reports.
5) Skilled at using scientific rules to solve problems.
6) Ability to understand current literature in tumor biology/biochemistry/transgenic animal techniques related to the current project/work within the lab.
7) Ability to design experiments.
8) Ability to perform experiments following laboratory protocols.
9) Skilled in basic molecular and cell biology techniques.
10) Ability to operate laboratory equipment.
11) Ability to perform basic calculations.
12) Good organizational and communication skills.
13) Ability to perform basic computer work (MS Office, Photoshop, DNA/protein analysis, statistical programs).
14) Ability to maintain well-organized laboratory notebook to document experiments and results.
15) Knowledge of laboratory animal housekeeping, breeding colony management, health assessment, cell/tissue transplantation and other techniques.

Behavioral Competencies
1) Technical skills – Strives to continuously build knowledge and skills; pursues training and development opportunities; assesses strengths, weaknesses, and shares expertise with others.
2) Problem solving – Gathers and analyzes information, identifies problems, develops alternatives, uses reason even when dealing with emotional topics and works well in groups and resolves matters timely.
3) Internal & External Customer Service – Manages difficult or emotional matters with objectivity and openness; responds promptly to needs and requests for service and assistance; meets commitments and dates, notifies others if change occurs; provides, solicits and applies feedback; and builds goodwill through balanced contributions, positive approaches, and demonstrating respect and support of others.
4) Communications – Demonstrates group presentation skills, participates in meetings, listens and gets clarification, is responsive and speaks clearly and persuasively in positive and negative situations. Writes and edits clearly and informatively, varies style to meet needs, presents numerical data effectively, and is able to read and interpret written information.
5) Organizational Support – Aligns work and develops strategies to meet organizational goals; seeks to improve and promote quality; demonstrates accuracy and thoroughness; and upholds organizational expectations and values.
6) Self-Management – Responds to management direction; resourcefully adapts to changes in work environment and circumstances; responds well under pressure and manages competing demands; takes calculated risks, asks for and offers help when needed; exercises discretion and sound judgment; is tactful and responsible for own actions. Adheres to work schedule and complies with leave management responsibilities.

Preferred Qualifications & Credentials
Education and/or Experience
1) A master’s degree is preferred.
  • AA/EOE/Minorities/Females/Vet/Disability/E-Verify Compliant Employer.
  • Please view Equal Employment Opportunity Posters provided by OFCCP here.
  • The contractor will not discharge or in any other manner discriminate against employees or applicants because they have inquired about, discussed, or disclosed their own pay or the pay of another employee or applicant. However, employees who have access to the compensation information of other employees or applicants as a part of their essential job functions cannot disclose the pay of other employees or applicants to individuals who do not otherwise have access to compensation information, unless the disclosure is (a) in response to a formal complaint or charge, (b) in furtherance of an investigation, proceeding, hearing, or action, including an investigation conducted by the employer, or (c) consistent with the contractor's legal duty to furnish information.
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